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Courses and Seminars related to CryoEM

At the NYSBC

Workshop in Dual-Beam Scanning Electron Microscopy at the New York Structural Biology Center

This workshop will introduce the new Helios 650 Nanolab microscope at NYSBC and illustrate its capabilities for producing large scale (10um x 10um x 10um) 3D reconstructions through resin-embedded tissue as well as for preparing frozen, whole-mount cells for electron tomography. In the literature, this method is alternately referred to as Focused Ion Beam (FIB) milling and Ion Abrasion SEM.

  • January 26, 2012
  • 1-5:30 pm
  • NYSBC Seminar Room A-11
  • To register, please RSVP to cem@nysbc.org

Agenda

  • 1:00 pm - David Stokes/Bill Rice (NYSBC): Introduction to the Dual-Beam SEM Microscope
  • 1:45 pm - Dave Hall (Einstein College of Medicine): Special Sample Procedures for C. Elegans; Enhanced en bloc Staining
  • 2:30 coffee
  • 3:00 - Gavin Murphy (NCI/NIH): Methods for Reconstructing HIV-infected T cells: Correlative LIght & FIB-SEM Microscopy and Image Processing
  • 3:45 - Peijun Zhang (Univ. of Pittsburgh): Cryo-FIB and correlative approaches for 3D imaging of native cells
  • 4:30 - Paul Anzalone (FEI Applications Specialist) tour/demo of dual-beam microscope and reconstruction software
  • 5:30 - Reception

NYSBC is located at the intersection of 133rd St. and Convent Ave on the southern end of the campus of City College of NY. Take the ABCD train to 125th St. and walk up Convent Ave. Or take the 1 train to 137th and walk down Broadway to 133rd St.

CemWorkshopRegistrants

EM Journal Club (If you are interested in presenting a paper please email cem@nysbc.org)

Past Journal Club Articles

Semester-long Course: "Cryoelectron Microscopy of Macromolecular Assemblies"

This comprehensive course will cover the theory and practice of solving molecular structures by electron microscopy. We will start with optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis, and 2D crystallography. We will end with a discussion of map interpretation and molecular fitting. Two weeks are devoted to practical sessions giving students hands-on experience with a variety of software packages for image reconstruction.

Offered every fall from September - December. 2011 Course Announcement, view flier, download flier

Local Cryo-EM Workshops and Meetings

National & International Cryo-EM Meetings, Workshops and Practical Courses

2nd UB Practical course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS)

5-7 June 2012 / 12-14 June 2012

Institute of Anatomy, University of Bern, Switzerland

Organiser : Benoît Zuber

Instructors: Benoît Zuber, Daniel Studer, Dimitri Vanhecke

The objective of the course is to teach the participants the practical skills necessary to successfully apply CEMOVIS in their laboratories. Essential background theory of CEMOVIS will be given and most of the time will be spent practicing high-pressure freezing, cryo-sectioning, and low-dose TEM imaging. 1 high-pressure freezing machine, 3 state-of-the-art cryo-ultramicrotomes and 1 cryo-electron microscope will be dedicated to the course. The 3-day course is given twice, each time with a maximum of 4 participants so that they can be actively practicing at any time.

The course is intended for scientists whose research projects will benefit from the use of CEMOVIS. Experience in either cryo-electron microscopy or ultramicrotomy of resin-embedded specimens is a prerequisite.

For details and registration please visit http://www.ana.unibe.ch/event/cemovis/

EMBO practical course on cryo-EM and 3D image processing

The EMBO practical course on cryo-EM and 3D image processing will take place this year from 26th August-2nd September at EMBL in Heidelberg. As in the past, the course will provide in depth theoretical and practical training, with the focus on image processing for 3D reconstruction. Registration is now open.

Further details, including a list of confirmed teachers are available here: http://www.embl.de/training/events/2012/CRY12-01/index.html

6th International Conference on Structural Analysis of Supramolecular Assemblies by Hybrid Methods (March 14-18, 2012 in Lake Tahoe, California)

The abstract submission deadline for the 6th International Conference on Structural Analysis of Supramolecular Assemblies by Hybrid Methods has been extended. Abstracts submitted on or before February 28 will be considered for short oral presentations at the meeting. Abstracts for posters only will continue to be accepted through March 11th. The registration fee is $375 before January 31st. If space is available, we will allow delegates to register right up until the start of the conference, but the “walk in” registration fee is $425/delegate.

The symposium will be held from March 14-18, 2012 in Lake Tahoe, California at the Granlibakken Conference Center. We invite you to visit our Symposium website at:

http://www.hybridmethodsconference.com/

where you can register for the Symposium, submit an abstract, and view the program.

We have recruited an outstanding panel of speakers including two Keynote Speakers: Wes Sundquist (University of Utah), and Susan S. Taylor (UCSD). Also presenting a special "Vision" talk on the future of Hybrid Structural Methods will be Michael Rossmann (Purdue University).

1st UB Practical course on Cryo-Electron Microscopy of Vitreous Sections (CEMOVIS) (20-24 June 2011)

  • 20-24 June 2011
  • University of Bern, Switzerland

To register please send a curriculum vitae and a cover letter briefly describing your current or future research and how it is going to benefit from CEMOVIS to daniel.studer@ana.unibe.ch or bzuber@mrc-lmb.cam.ac.uk.

PARTICLE Workshop (July 2, 2011)

In conjunction with the Apollo release of PARTICLE (www.image-analysis.net/EM), I.A.N. will host a training workshop to introduce the main features of the program upgrade. The topics of this one-day workshop include:

  • An overview of single-particle reconstruction
  • The design of PARTICLE: architecture and functionality
  • Data processing in PARTICLE: from concept to practice
  • Data Clinic for individual users coming with real single-particle data

The workshop will be presented on July 2, 2011 in Cambridge, Massachusetts, USA. If you are planning to attend, please register online at:

www.image-analysis.net/EM/Workshop/registration.htm

3rd International High Pressure Freezing Meeting (13-15 July 2011)

5th International UVM Practical Course on Three-dimensional Cryo-Electron Microscopy of Single Particles (July 24-31)

This international course will teach the principles of three-dimensional reconstruction of single particles from cryo electron micrographs. The course will include in-depth teaching of the basic theoretical principles of single particle analysis, discussions and demonstrations of experimental procedures, and six hours per day of hands-on processing of data sets from electron micrographs of single particles. Participants will work in groups of two. Each group will have one instructor, who will provide step-by-step guidance through the complete three-dimensional reconstruction process and offer additional explanations and support. The course aims to provide participants with a strong practical and theoretical background that will enable them later to use these techniques in their home laboratory.

Application deadline: April 15th

"Practical Course on Correlative Light Electron Microscopy" in Bristol, UK. (July 25 -29, 2011)

Not just Tomography but part of almost all the CLEM workflows nowadays, so that will also definitely be covered. Website

IMOD Software Workshop for Biological Specimens (August 2-5)

  • University of Colorado, Boulder
The workshop will cover dual-axis plastic section tomography, joining of serial section tomograms, tomogram flattening, segmentation, and analysis. The course will also cover cryo-tomography, CTF correction, and gold erasing. PEET (3D volume averaging) will NOT be covered. There will be time at the end of the workshop for participants to work on their own data that they bring from home. The workshop will be organized with both lectures and hands-on practicals. A limited number of applicants (20-25) will be selected due to space considerations. Priority will be given to applicants who are lab trainers so they can train other members of their lab. A $225 registration fee will cover course materials and lunches. Participants must pay for their own transportation, lodging, and food other than lunches. Do not send the registration fee until you have been selected as a participant for the workshop. The online application below will be available until 5PM (MST) on April 22, 2011. All applicants will be notified of their status by 5PM (MST) on April 29, 2011. survey

Microscopy and Microanalysis 2011 (7-11 August 2011)

Workshop in Electron Crystallography (August 15-19 2011)

  • Seattle, Washington
  • Course Website
  • Topics:
    • 2D crystallization of membrane proteins
    • Specimen preparation for electron microscopy
    • Collection of images and electron diffraction
    • Fourier theory as it relates to crystallography
    • Use of software for analysis of planar and helical crystals
      • MRC image2000
      • 2DX
      • IPLT
      • SPARX
      • EMIP

EMBO practical course on image processing for cryo-electron microscopy (30 August - 9 September 2011)

  • Birkbeck, Malet Street, London WC1E 7HX, United Kingdom
  • Course Website
The course will cover:
  • Basic concepts of Fourier analysis
  • Principles of TEM image formation and contrast transfer
  • Image acquisition and pre-processing
  • Contrast transfer function correction
  • Symmetry and alignment
  • Multivariate statistical analysis and eigenimages
  • Determination of orientations for 3D reconstruction
  • Angular reconstitution
  • Methods of 3D reconstruction
  • Icosahedral reconstruction
  • Electron crystallography
  • Helical reconstruction
  • Interpretation: Atomic structure fitting into EM maps

Extreme Resolution Cryo-SEM Workshop (October 2011)