The JEOL 2100F operates with an accelerating voltage of 200kV. It is equipped with a Schottky field emission gun and an array of three condenser lenses, which adds versatility to the illumination system with respect to previous designs. A set of apertures placed at the focal plane of the objective lens, and an extra set of apertures is located below the focal plane to privide additional contrast to biological specimens.

Two cameras are installed on this microscope for high end applications. A Tietz fast scan camera allows the search of suitable areas of the specimen under low magnification, low illumination conditions, as well as focusing at high magnifications prior to exposure. The high quality data is recorded either on film plates, or on a 2kX2K CCD Tietz camera with a 24um pixelsize.

The program SerialEM is installed on all the instruments at the NYSBC to provide the capability to collect tilt series for tomographic reconstructions, on a common interface that can be easily learned by the affiliated users.

Specifications for this instrument, as well as operational specifics are available to affiliates of the NYSBC at http://www.nysbc.net/twiki/bin/view/Main/CryoEM.

The images on the right hand side correspond to frozen hydrated influenza virus (top), and cryo-negative stained tobacco mosaic virus, collected both on the J2100 instrument. Click on the pictures to see a larger version.

Instructions for use

Basic Instructions for use

Information and Calibrations