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At the NYSBC
- Tues and Thurs Sept 16 - Dec 7 2010
Cryo electron microscopy in combination with image analysis is increasingly powerful in producing 3D structures of individual molecules and large macromolecular complexes that are unapproachable by other methods.
This comprehensive course will cover the theory and practice of solving molecular structures by electron microscopy. We will start with optics, sample preparation and a basic mathematical description of diffraction before moving into a detailed exploration of the three main methods of structure determination: tomography, single particle analysis, and 2D crystallography. We will end with a discussion of map interpretation and molecular fitting.
EM Journal Club
EM Journal Club is on summer break. We will resume the bi-weekly lecture series on Thursdays beginning Sept 16
National & International Cryo-EM Meetings, Workshops and Practical Courses
- August 29 - September 5, 2010
- EMBL Lab
- Heidelberg, Germany
The EMBO practical course on cryo-EM and 3D image processing will take place this year from 29th August-5th September at EMBL.
Registration is now open, and will close on 10th May.
As in the past, the course will provide in depth theoretical and practical training, with the focus on image processing for 3D reconstruction.
Further details, including topics covered and a list of confirmed teachers are available here:
http://www.embl.de/training/courses_conferences/course/2010/CRY10-01/index.html
Best wishes,
John Briggs
- September 8-19, 2010
- Rio de Janeiro, Brazil
After three successful schools in this series, we are happy to
announce the Fourth Brazil School for Single Particle Cryo-EM. This
school will take place prior to the International Microscopy Congress 17 which
will be held in Rio de Janeiro.
This single-particle school is
an intensive hands-on course/workshop, in which theory and practice
are tightly intertwined. The spectrum of topics covered suits
attendees that are entirely new to the field as well as those for whom
the technique is of growing importance and who wish to acquire an
in-depth understanding of the field. Topics range from data collection
and three-diemensional reconstruction techniques to the interpretation
of fitted X-ray structures into cryo-EM maps. The final days of the
conference will be devoted to more advanced topics such as dealing
with heterogeneous data sets ("4D" cryo electron microscopy) and
high-resolution refinements.
The attendees are expected to bring
along a modern notebook computer onto which all necessary software
will be installed. Most processing will take place in the context of
the IMAGIC software system but other software packages will also be
available. Cryo-EM data sets will be distributed for processing during
the course. Students may bring along their own data sets for
processing and discussion.
- October 12-13, 2010
- Lister Hill Auditorium
- NIH campus
- Bethesda, Maryland
Characterization of the interactions of viruses with their host cells is a problem that spans over six orders of magnitude in scale from the atomic level (0.1nm) to the cellular lever (10’s of microns). This range of scale is beyond the scope of any currently available imaging methods and has led to the development and application of innovative hybrid approaches combining x-ray crystallography, electron microscopy, electron tomography, and optical microscopy to probe virus and cellular interactions at a single particle level in vitro, in fixed cells, and even in live cells.
The use of hybrid approaches is rapidly developing. These approaches have been driven by improvements in instrumentation, sample preparation methods, and image processing methods. The application of these hybrid methods has begun to produce new findings that would not have been possible even five years ago. These new findings have clear relevance to the biology of viruses, the biology of the cell, and to human health. The broad range of expertise required to apply these hybrid methods, and the rapid pace of the development of methods and results, place increasingly heavy demands on communication and education, and create an urgent need for an forum to promote discussion among investigators in different disciplines who might not otherwise meet, in a format that will promote the continued development and expanded application of these highly promising approaches.
The objectives of the workshop will be:
- to provide an overview of the field that is rapidly evolving;
- to foster discussions on combining various imaging technologies and the roles of each imaging technologies as applied to virus infection;
- to explore how imaging technologies can be applied for developing innovative methods for diagnostics and antivirals for therapeutic intervention;
- to promote communication between labs working in this area.
In addition to invited talks, there will be poster sessions selected from submitted abstracts. We encourage you to submit abstracts.
Please click here to submit abstract for poster sessions.
Deadline for submitting abstract is August 25, 2010.
There is no registration fee, but registration is required for attending the workshop. Due to the space limitation, those submitting abstract may be given a priority for attending the workshop. Registration is open until space is filled. Please register early to ensure you will have a reserved space. Please click here to register.
2011
3rd International High Pressure Freezing Meeting
- July 13-15, 2011 - London England
Microscopy and Microanalysis 2011
- August 7-11, 2011 – Nashville, TN
-- DavidStokes - 02 Oct 2009
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