TecnaiAdvanced

Sample Insertion

Access plate is jammed by lifting screen and looking through chamber viewing port.
Turn off pneumatics before attempting to dislodge or risk of losing finger

If a software problem prevents column valves closing; pressing Close V7 button (located on left side behind viewing screen) to close manually
- Because of a bug, the TEM software is not happy if you press it an odd number of times, so after restart of computer, press it once more
- If it was pressed an odd number of times, then HT and FEG will turn off upon opening valves. If this happens, press it once more, restart HT and try again.

Make sure house chiller outside is operational. Check with Mike Goger, Dan Murphy or Kaushik Dutta.
Restart the F20's chiller in B-1
- Remove the front panel and press reset button marked with an arrow.
- Add ice to the water bath to start recirculation (temperature must be <70F to start)
- Check insulated pipe to make sure cool water is going through the lines
Optionally, take this opportunity to reboot the TEM computer
You will need to restart:
- The CCD Camera (physically turn it on)
- The vacuum system
- The HT
May need to restart TEM Server; probably best to simply reboot PC if this is the case

After a computer restart, TEM Server may not restart automatically (this seems to be fixed in new software as of March 2012)
Manually restart it as follows:
- Go to Start menu > Programs > Administrative Tools > Services
- Make sure TEM Server Status: started (right click and choose start if not)
Open the Tecnai User Interface
- In High Tension panel move slider as high as software will allow (towards 200kV) to keep lenses warm

Make sure vacuum on
- The banner on the Vacuum Expert Panel will give you the current status of the vacuum system.
- If red, manually turn on vacuum - White Vac. button (do not trust if it is lit). You should hear valving and Vacuum (Expert) panel status should read "Starting up"
Make sure White HT button is lit. If not lit, press it.
Go to Setup > FEG control Panel
- If "Operate" button is unlit in FEG Control turn on "Power"
- Warm Start if under an hour, Cold Start if over an hour.
- Choose Cold start if you do not know how long it was off. The warm or cold start determines how fast FEG reaches targets.
Select High Tension in the High Tension panel
- Move High Tension to 200 kV, if not already. Check Measured HT: 201 kV
- Make sure Gun lens: 3 (make sure by toggling between 2 and 3) and Extraction voltage: 3950. Click on arrow to ramp from 3400 to final target of 3950.
- Make sure desired condenser aperture in

Center condenser aperture
In alignment panel follow Gun alignments
- Gun Tilt - may be more than 1 bright spot, make sure not too close to aperture edge
- Gun Tilt Pivot Points
- Gun Shift - may have to do it several times
- Check condenser aperture alignment again and condenser astigmatism
- Spot size-dependent Gun Shift (optional) - spot 1 usually off
Do a final round of direct alignments
- Beam tilt pp X and Y
- Beam shift
- Rotation center.

Condense beam to point
- Beam may not be round at crossover, this is the image of the FEG tip
- 5mm circle = 10nm
- You want the majority of your beam to fit within the 5mm circle

If not make sure CCD Blank Enable box present. CcdBlankEnable
- Activating Tecnai Low Dose usually disables this box; must be re-enabled
Check pre-exposure time
- Open "Pre-exposure" shortcut on Desktop
- Under "Shutter Box Control" make sure pre-exposure time is 0 ms

If you are told by NYSBC or FEI personel to shutdown the microscope
- Press the Red Button on the right panel
  - This activates the "Standby Vacuum" which keeps IGP's on while shutting down the DP
  - Both red and green lights will remain on

-- BillRice - 29 Sep 2007