JEOL 3200 Microscope Parameters

wavelengths

300kV: 0.01969 A

objective lenses

f= 4.4 mm
Cs = 4.1 mm
Chromatic aberration = 3.4 mm

Aperture Sizes

Condenser : 150 um, 70 um, 50 um, 20 um
Objective : 50 um, 50 um, 40 um, 40 um
Selected Area: 100 um, 50 um, 20 um, 10 um
Entrance Aperture : 120 um, 60 um, 40 um, 20 um

Energy Filter

Slit width at 10 eV : 60 mm on film
Slit width at 2 eV: 25 mm on film

Expected Values

Dark Current: 104 uA (12-5-11)
Emission Current: 179 uA (12-5-11)
Column / PiG1: 33 uA (12-5-11)
Camera / PiG3: 30 uA (12-5-11)
PEG1: 1.5 *10^-5 Pa (12-5-11)

Ice Thickness

The ice thickness was measured by punching a hole on the ice at 30 degrees (tilt) and measuring the length of the hole at -30 degrees (tilt). Using a collection semiangle of 45mrad, and assuming that the ice scatters as Carbon, the value that DM gives for the thickness is roughly half the actual thickness of the ice.

CCD information

Size of camera : 4096 x 4096 pixels
Pixel size: 15 um
Post-film mag : 1.40
Gain in SerialEM and Digital Micrograph : 29 counts / electron

Part number for replacement balloons

part #780 208 463
Used for filling liquid helium

CCD : Signal to noise ratio in comparison to film

Magnification Calibration: Jeol 3200FSC

Magnifications calibrated using 2160 cross grid using method described in SerialEM documentation

Film Magnification	CCD Magnification	Unbinned Pixel Size (A)	2X Binned Pixel Size (A)	Angle of Tilt Axis	Calibrated Film Magnification
LM 100	143	1046	2093	206.3
LM 120	190	791	1583	212.5
LM 150	248	606	1212	219.8
LM 200	313	479	957	221.7
LM 250	360	417	833	222.6
LM 300	426	352	703	222.9
LM 400	556	270	540	223.9
LM 500	679	221	442	224.5
LM 600	808	186	371	224.8
LM 800	1,057	142	284	226.0
LM 1,000	1,302	115	230	224.7
LM 1,200	1,574	95.3	191	223.1
LM 1,500	1,965	76.3	153	226.8
LM 2,000	2,650	56.6	113	229.8
LM 2,500	3,442	43.6	87.2	234.9
LM 3,000	4,300	34.9	69.8	238.6
MAG 2,000	2,750	54.5	109	155.6
2,500	3,439	43.6	87.2	154.7
3,000	4,160	36.1	72.1	154.9
4,000	5,574	26.9	53.8	155.0
5,000	6,794	22.1	44.2	125.7
6,000	8,050	18.6	37.3	126.0
8,000	10,841	13.8	27.7	126.2
10,000	13,798	10.9	21.7	125.2
12,000	16,598	9.04	18.1	125.8
15,000	20,741	7.23	14.5	126.4
20,000	26,806	5.60	11.2	126.1
25,000	33,962	4.42	8.83	127.2
30,000	40,859	3.67	7.34	127.3
40,000	54,074	2.77	5.55	125.6
50,000	68,165	2.20	4.40	126.0
60,000	81,221	1.85	3.69	125.8	59,717
80,000	106,738	1.41	2.81	125.8
100,000	132,895	1.13	2.26	125.8
120,000	157,146	0.95	1.91

* Overall CCD to film factor: 1.40

-- BillRice - 28 Sep 2007