JEOL 1230 Instructions

Specimen Insertion

Flip toggle switch to "pump" (G)
Line up pin on holder with goniometer(G)
Insert specimen holder to first sample stop (marked line indicates correct depth)
- You will hear a click
- Orange light will go on
Monitor Vacuum condition:
- Go to "FR Panel" "monitor" "vacuum conditions"
- When SPC value is <50 insert holder by turning the holder slowly clockwise 1/8 turn to the 1st stop
Slowly allow the vacuum to pull the holder in
Grasp holder firmly then turn the holder slowly clockwise 1/4 turn to 2nd stop
Slowly allow the vacuum to pull the holder in
Wait for green light to come on before turning on beam

At 5000x
Use "Brightness" knob to condense beam on function box(F)
press "Beam Shift" (F)
Right click mouse button and drag the beam to central position
Spread Beam (over clockwise) with "Brightness" knob (F)
If it does not spread evenly, center with the condenser aperture x-y controls
condense beam with "Brightness" (F)
If beam is not centered, repeat
The beam should expand/ contract concentrically around the beam centre as the brightness is adjusted

Switch the beam is off
Switch the toggle to "Air" (G)
Monitor Vacuum condition:
- Go to "FR Panel" "monitor" "vacuum conditions"
Pull out specimen holder to 1st stop
Rotate anti-clockwise 1/4 turn
Pull out the specimen holder to the 2nd stop
Rotate anti-clockwise 1/8 turn
Wait for the airlock "SPC" to vent
Gently remove the specimen rod (G)
- If the use of force is necessary the SPC is not vented

To do a low-mag montage, go to 100X or 80X, fully expand beam, take a test Record image to be sure images are OK
Go to Navigator menu, choose to Open
Go to Navigator menu, choose Setup Full Montage
Choose to save all header info, save file as FILENAME.st
Increase overlap percent to 20 (3X3 images for full montage at 100X)
Click Start on montage menu
Montage will begin. If it appears to get stuck, quickly toggle mags
When done, click YES to make a new map
Try checking and unchecking Treat as very sloppy montage -- sometimes it is better, sometimes worse
Go to File menu, click close to close the file for writing (do not want to add to this file)

First map is registration 1
Make a new montage, either at a different mag or at a different grid orientation
Follow instructions above, but before you click start, CHANGE CURRENT REGISTRATION TO 2
Make the new map -- you now have old map in registration 1 and new in registration 2
Need at least 3 points to align the maps (determine a shift/rotation matrix)
Choose three obvious points, click Add Points, and add points. When done, click stop
Mark each point as a Registration point, numbered 1, 2 and 3
Re-open first map by double clicking
CHANGE CURRENT REGISTRATION BACK TO 1
Click Add Points, and add the three corresponding points on the old map
Mark these as Registration Points , again 1,2,3, IN THE SAME ORDER AS ON MAP 2
Change current registration back to 2, and re-load new map
Under Navigator menu, choose Transform items
Map 1 is now transformed onto map 2

Change to desired new magnification (say 4000X )
Take Record image to test beam and image settings
In low mag map, choose Add Polygon and outline a grid square
With Polygon highlighted, go to Navigator menu and choose Setup Polygon Montage
Increase overlap to 15%-20%, and also choose to Move Stage
Change current registration as described above, and click start
When polygon montage is done, create a new map and align it to old map as described above
DO NOT CLOSE THE NEW FILE
In low mag map, decide which squares are to be acquired, and add a point in the middle of each
Mark each new point as ACQUIRE
Under Navigator menu, choose Acquire Areas
A polygon montage, centered at each point, will now be acquired.

* Set ALLOWTOPICVIEW =

-- KdDerr - 13 Jun 2006