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F20 Daily Checklist

• Cool microscope cold finger
• Insert holder to first stop
  • Do not insert holder
• Open colum valves
  • check for beam

• Start Low Dose and click through systematically to "exposure" mode
  • Load Lose Dose file you will be using

Operation Parameters for alignments

• 3850-3950 extraction voltage
• Gun Lens 3 (make sure it is gun lens 3 by toggling between 2 and 3.)
• 50 micron condenser aperture (check white board, for now it's position #2)

Gun tilt (check)

• Magnification 10kx
• Check exposure time without the small screen
  • Around 2 sec is appropriate
  • 2 sec exposure means 1 nanoAmp of current
    • if the exposure time is not close to 2 seconds double check for correct operation parameters
    • If the operation parameters are correct adjust Gun Tilt
• Location: tune/alignments/gun
• Adjust the gun tilt alignment to locate the brightest spot
• After adjusting Gun tilt:
  • If exposure time is over 2 sec increase the extraction voltage and readjust gun tilt
  • If exposure time is less than 1.4 sec lower the extraction voltage and readjust gun tilt

Condensor Stigmation

• Spot size 2
• Magnification 250kx
• Condense beam to 30mm size
• Press stigmator button
  • select condensor
• Use multifunction knobs to make beam round

Check image of FEG Tip

• Condense beam to point
  • Beam may not be round at crossover, this is the image of the FEG tip
  • 5mm circle = 10nm
  • You want the majority of your beam to fit within the 5mm circle

Flatfield Tietz camera

-- KdDerr - 10 Oct 2006