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Dual Tilt axis tomography on the JEOL-3200FSC

New - How to use the dual tilt holders of the 3200

• The JEOL-3200FSC comes with two sets of cartridges: single and double tilt
• The double tilt cartridges show a smaller area of the specimen, ~1mm in diameter.
• The spacer and the clip ring are in one single piece in these cartridges.
• The same tool is used for loading the cartridges and loading is done in a similar way.

1. When the cartridge is loaded, it will be in a configuration such that it can only be rotated counterclockwise.
2. Do a low mag montage or whatever you need to recognize the same area of interest afterwards.
3. Go to the area of interest to collect the first tomographic series.
4. Mark the current location on the JEOL software, by clicking on the spc window.
5. Reset the holder to the zero position.
6. Put the "L" cartridge on the holder that says "jig holder" and insert it into the chuck (after cooling it!)
7. Once the jig is in the chuck, place the chuck spacer so that the jig stops at the proper location
8. Retract the jig and remove it from the chuck
9. Click on the spc window the icon for rotation of the holder
10. Click the "recall" button. This will take you to a place close to the area of interest.
11. Re-identify area of interest and proceed to data collection.

-- RubenDiaz - 31 Mar 2009