Interested in EM?
Using the NYSBC
Principles & Protocols
NYSBC Equipment
Seminars & Courses
Publications associated with CEM at NYSBC
Jobs Available
Microscope Schedule
Logbook
NYSBC Intranet
NYSBC Contacts
Cryo-EM home
NYSBC home
NMR Facility
X-Ray Facility
Protein Production Facility
Electron Crystallography Center
Cryo-EM Site map
|
Principles & Protocols
Principles - Carbon Coating
The best method to use for carbon coating is an on going debate. Best to find a method thatthat works and stick to it.
Substrates
- Pure carbon
- Nitrocellulose
- Formvar
Deposition Methods
Carbon Thread
- Reproducible results
- Varying thread thicknesses available
Carbon Rod
- Results not 100% reproducible
- Graphite vs. pure carbon debate - graphite is softer than carbon meaning that less energy is required to evaporate graphite than carbon.
- Flat vs. pointed rod debate
- carbon rod sharpening angles:

-- KdDerr - 29 Aug 2007
|