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About the EM Facility

We help researchers elucidate the intermolecular interactions and domain architectures of macromolecules within their native cellular assemblies. Our advanced instrumentation and staff expertise assist users to support the determination of protein structures by single-particle reconstruction and 2-D crystallography, as well as organelles and tissues by tomography and FIB/SEM.

News and Events

New Equipment: Direct Detectors at NYSBC TEM Detector
Two new direct detectors have been intalled at the NYSBC EM facilities. A Gatan K2 Summithas been installed on the JEOL 2100, and a Direct Electron DE20 has been installed on the JEOL 3200. These cameras are currently undergoing initial beta testing, but will be available for general use very soon.
New Publication: 2.8A Resolution Proteasome Structure 2.8A
Melody Campbell from the Scripps Research Institute and David Veesler from the University of Washington worked with Bridget Carragher, Clint Potter, and Anchi Cheng from the Electron Microscopy Group at NYSBC using single-particle cryoEM to produce images of protein structure detailed enough to be useful for drug development.
New Publication Mammalian Photoreceptors FIM-SEM Mouse Rod OS
Ya-Chun Hsu, Jen-Zen Chuang, and Ching-Hwa Sung from Weil Medical College have published a paper concerning hte mechanism through which rod photoreceptors in mammals maintain a balance between memgrane bound discs that are created and discarded daily.

EMG Forums:

Wednesday 3/18
Ben Bammes, Ph.D.
Dir. of Applications/Marketing, Direct Electron

Building Proteins in a Day: Efficient 3D Reconstruction for Cryo-EM

Notes:

The EMG area is currently undergoing construction. Before scheduling equipment or microscope time please email Bill or Ed for availability.


J3200
Helios FIB-SEM
Defraction
CysZ Protein

Microscopes and Detectors

  • JEM3200 FSC (300 keV, FEG), Direct Electron DE20
  • JEM2100 (200 keV, FEG), TVIPS CMOS CCD
  • JEM1230 (120 keV, FEG); Gatan CCD
  • Dual beam Scanning FIB/SEM

Applications

  • Single particle reconstruction
  • Electron tomography
  • Large scale serial reconstructions
  • In situ FIB milling plus tomography